Human ELISA Grade Type II Collagen

Cat No:C-0029

Description

Monomeric collagen can be used as an ELISA antigen to form a monolayer of collagen on the surface of the ELISA plate and quantify anti-collagen antibodies in the sample.

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Basic Information

Specifications	0.5 mg/mL x 1 mL
Synonyms	Highly purified type II collagen
Applications	Our ELISA grade collagen can be used as an ELISA antigen for quantitative anti-collagen antibody detection.
Form	0.5 mg/mL solution in 0.05 mol/L acetic acid
Source	Human sternal cartilage
MW	300 kDa
Purity	>99% by SDS-PAGE, free of pepsin and proteoglycans
Storage	It is stable for several weeks at 4°C under acidic conditions and for one year at -20°C under dark conditions, but gradually forms polymerized collagen and degrades under neutral conditions.
Shelf Life	1 year
Usage	When coating plates, take an equal amount of ELISA Grade Collagen and dilute it with cold 1X Collagen Dilution Buffer before use. The optimal collagen concentration for coating ELISA plates is 5 µg/mL, but may vary depending on the assay or type of plate used.

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