Porcine Type I Collagen-Cell Culture Grade

Cat No:C-0002

Description

Type I collagen, the main collagen in connective tissue and a major component of the interstitial matrix of many tissue types, is one of the most common natural materials used in 3D scaffolds.

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Basic Information

Specifications	4 mg/mL x 12.5 mL
Synonyms	Acid soluble porcine type I collagen solution
Applications	This product is used in cell culture to promote cell attachment, proliferation, migration and differentiation.
Form	Solution in 0.01 mol/L HCl
Source	Porcine
Endotoxin	Less than 10 EU/mL
Purity	>95% Type I collagen
Storage	4°C
Shelf Life	6 months
Usage	Plate Coating: 1. Dilute 4 mg/mL collagen with 50 to 100 μg/mL of 0.02 mol/L HCl. 2. Gently mix the diluted solution. 3. Add the appropriate volume of diluted collagen solution to the wells or plates. Note: Make sure the entire surface is coated. 4. Incubate for 1-2 hours at room temperature or 37°C. 5. Remove all solution. 6. Carefully rinse coated surfaces with medium or PBS. 7. To maintain sterility, store coated wells or plates at 2-8°C or air dry. Three-dimensional (3D) Gel Preparation: 1. Dilute collagen solution with an equal volume of cold sterilized PBS (final concentration 2 mg/mL). 2. Add the appropriate volume of diluted collagen solution to the wells or plates. 3. Incubate at 37°C for 30-60 minutes. 4. The gel can be stored at 2-8°C or dried while maintaining sterility.

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