CD BioSciences-GreenChemistry has set up an enzyme-catalyzed reaction technology platform that aims at designing and improving specific catalytic enzymes to suit the requirements of enzyme technology for different sectors. Thus, through this action, we assist our clients with custom-tailored enzyme purchases, to help them enhance their output and keep up with the green chemistry and sustainability ideals.
Enzymes are protein molecules whose presence as catalysts is characterized by great specificity and catalytic efficiency. These molecules can be natural catalysts and due to their properties, tend to enhance the reaction rates of biochemical processes by decreasing the energy that is needed to trigger a reaction. Enzymes can be manipulated to perform chemical reactions so they are considered mild catalysts. The use of enzymes requires less energy and eliminates the production of waste products that would usually be formed through a conventional method of applying chemical catalysts. Their application ranges from pharmaceuticals, agriculture, and food processes, thus showing their substantial benefits and strong support for the goal of green chemistry and responsible development.
Fig.1 Metamorphic fine-tuning of enzyme-catalyzed processes. (Yao & Hamelberg, 2024)
After years of independent research and development and technology accumulation, CD BioSciences-GreenChemistry has mastered the enzyme catalysis technology and constructed an enzyme catalysis platform that helps clients in different fields to synthesize target compounds efficiently.
Construction of Enzyme
First, we identify the specific chemical reaction to be catalyzed and analyze the reaction mechanism. Next, genes encoding the selected enzymes are isolated from nature or artificially synthesized and cloned into appropriate expression vectors. Targeted evolution techniques are also used to progressively optimize the performance of the enzyme to construct and optimize the enzyme for a specific application.
Fermentation of Enzyme
We select optimized or modified host microorganisms (e.g., Escherichia coli, yeast, etc.) and inoculate them into fermenters to start the primary fermentation process. Fermentation conditions are precisely controlled to optimize bacterial growth and enzyme expression. Fermentation is terminated by rapidly lowering the temperature or supplementing with inhibitors when maximum enzyme production is reached. The enzyme is then purified using centrifugation, precipitation, ultrafiltration, and chromatography.
Screening of Enzyme
We utilize high-throughput screening techniques to test the activity of a large number of candidate enzymes in microtiter plates or microreactors. Then, enzymes that meet the criteria and are catalytically active are screened by assays such as color reaction, fluorimetry, or occlusion analysis. For the enzymes to catalyze subsequent reactions efficiently, we perform detailed activity assays and kinetic parameter evaluations to determine the enzyme stability, the reaction conditions, and the substrate specificity of the enzymes.
Enzyme Catalytic Process Optimization
Depending on the specific reaction requirements, we offer specific process optimization. This includes evaluating the effect of different solvent systems on enzyme activity, screening auxiliaries and additives, and determining the optimal reaction time and temperature. The optimized process conditions are then applied on a pilot scale to verify their suitability for scale-up. During the implementation of the enzyme-catalyzed process, we provide real-time monitoring of the reaction to ensure that it meets the actual production requirements.
For certain specific reactions of chemical small molecule synthesis, we first conduct high-throughput screening from existing enzyme libraries to obtain enzymes that can catalyze the synthesis reactions, and then, based on the preliminary screening results and product targeting, we carry out process optimization in terms of chirality and conversion rate to improve the relevant parameters to achieve the synthesis of chiral compounds and the purpose of chiral disassembly.
Fig.2 The process of developing an enzyme-catalyzed process. (CD BioSciences-GreenChemistry)
CD BioSciences-GreenChemistry's enzyme-catalyzed reaction technology platform offers high-performance, green, customized solutions, and rapid screening capabilities to help clients improve reaction efficiency, reduce production costs, and achieve sustainable development. We always welcome clients to contact us at any time to explore the unlimited possibilities of enzyme catalytic technology.
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Our products and services are for research use only and cannot be used for any clinical purposes.