Actinotalea fermentans-Y959-4

Actinotalea fermentans Y959-4 is a gram-positive actinobacterial strain belonging to the family Cellulomonadaceae and the genus Actinotalea. It has a key alternative English name: Cellulomonas fermentans (the original classification before taxonomic reclassification in 2007).
First isolated from a municipal dumping ground in Montaubert, Essone, France, in the early 1980s, this strain was misclassified for 25 years before being formally reclassified into the novel genus Actinotalea based on polyphasic taxonomic evidence. Its type strain is DSM 3133(T), with additional deposit identifiers including ATCC 43279(T), CFBp 4259(T), and JCM 9966(T).
Key taxonomic and biological characteristics of the strain include:
Non-motile, non-sporulating, and rod-shaped (coryneform) cellular morphology.
Strictly aerobic respiration and slight halophily, with optimal growth in 2-4% (w/v) NaCl.
A high DNA G+C content of 75.8% and peptidoglycan of the A-type cross-linkage with L-ornithine as the diamino acid.
Predominant fatty acids of anteiso-branched and straight-chain types, with menaquinones MK-10(H4), MK-9(H4), and MK-8(H4) as major isoprenoid quinones.

1. Select the appropriate culture medium according to the characteristics of bacteria; 2. Take out as soon as possible after culture and put it into the refrigerator for storage, pay attention to the storage temperature of different bacteria, such as fungi, Vibrio cholerae, etc. need to be stored at room temperature; 3. Record the results of the identification of the strains, including growth, colony characteristics, staining reaction, etc. when preserving the strains; 4. The strain is divided into two sets of storage, one set is used for the preservation of the transmission, and one set is used for the experiments. Regular seed transfer, every 3 generations of identification.

Produces xylanosome-like multienzyme complexes (MECs), which are nano-scale hemicellulose-degrading systems with a particle diameter of approximately 113.2 nm.
Exhibits broad carbon source utilization, enabling growth on numerous organic substrates.
Features a distinct taxonomic lineage within the suborder Micrococcineae, with limited close relatives, making it a unique microbial resource.
Demonstrates specific fermentation metabolism, producing acetic acid, ethanol, formic acid, succinic acid, CO₂, and occasionally lactic acid.

Biomass conversion: Used in co-culture systems with genetically engineered yeast to convert lignocellulosic biomass into value-added compounds such as methyl halides, addressing limitations of acetate accumulation.
Hemicellulose degradation: The xylanosome complexes enable targeted breakdown of hemicellulosic components in plant biomass, supporting biofuel and biopolymer production.
Microbial biotechnology research: Serves as a model organism for studying unique enzymatic systems, taxonomic diversity, and synthetic symbiotic relationships.
Industrial fermentation: Potential for development in biorefineries to produce organic acids and bioethanol from renewable feedstocks.
Environmental biotechnology: Explorable for waste valorization, given its origin from municipal dumping grounds and ability to utilize complex organic substrates.

Customers interested in Actinotalea fermentans Y959-4 may also seek other hemicellulose-degrading microbial strains, xylanosome-enriched microbial extracts, cellulolytic actinobacterial isolates, and synthetic microbial consortia for biomass conversion. Additional relevant products include actinobacterial strains from the suborder Micrococcineae, multi-enzyme complexes for lignocellulose degradation, and halotolerant aerobic fermentative bacteria. If you are interested in related products, you can contact us directly or request product customization services.