Microsatellite MarkersCD BioSustainable offers cutting-edge microsatellite marker analysis services for a wide range of applications in conservation biology, wildlife management, fisheries, agriculture, and more. Our expertise in molecular ecology and genetics, coupled with state-of-the-art laboratory facilities, enables us to deliver accurate and reliable results that empower researchers, conservationists, and industry professionals to make informed decisions.
Fig 1. Microsatellites as Molecular Markers for Aquatic Animal Populations (Roman W., et al., 2023)
Microsatellites, also known as Simple Sequence Repeats (SSRs) or Short Tandem Repeats (STRs), are short, repeating sequences of DNA found throughout the genomes of most organisms. These sequences consist of 2-6 base pair motifs repeated multiple times, and the number of repeats at a specific locus can vary significantly between individuals and species. This high degree of polymorphism makes microsatellites ideal genetic markers for a variety of applications.
Microsatellite marker analysis service involves the following steps:
DNA Extraction: High-quality DNA is extracted from your samples using appropriate methods depending on the sample type (e.g., tissue, blood, hair, feathers, scales, etc.).
PCR Amplification: Specific primers designed to flank the microsatellite loci of interest are used to amplify these regions from the extracted DNA using Polymerase Chain Reaction (PCR).
Fragment Analysis: The PCR products are analyzed based on their size using capillary electrophoresis. Since the number of repeats varies between individuals, the amplified fragments will differ in length, generating unique profiles.
Data Analysis: The resulting data is analyzed using sophisticated software to determine allele frequencies, genetic diversity, population structure, and other relevant parameters depending on your research question..
We offer analysis of various types of microsatellites, including:
Our microsatellite marker analysis service can be applied to a diverse range of fields, including:
Assessing genetic diversity and population structure of endangered species, identifying cryptic species and evolutionary significant units (ESUs), monitoring population connectivity and gene flow, detecting hybridization and introgression. Informing conservation management strategies.
Identifying fish stocks and populations, tracking migration patterns and connectivity, assessing the impact of fishing pressure on genetic diversity, supporting sustainable fisheries management practices.
Monitoring population dynamics and demographics, identifying individuals for wildlife forensics and anti-poaching efforts, studying mating systems and reproductive success, assessing the impact of habitat fragmentation and climate change.
Characterizing crop varieties and germplasm diversity, assisting in breeding programs for improved yield and disease resistance, assessing genetic diversity in livestock for conservation and production, tracing the origin and authenticity of agricultural products.
Identifying and tracking bacterial and fungal pathogens, studying microbial diversity and community structure, monitoring the spread of antibiotic resistance.
We specialize in leveraging the power of microsatellite markers to provide you with the most accurate and reliable biological species identification services. Our comprehensive suite of services includes:
By choosing our microsatellite markers services, you are partnering with a company that is at the forefront of genetic research and species identification. Contact us today to discover how our services can add value to your projects.
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Our products and services are for research use only and cannot be used for any clinical purposes.
