Conventional siRNA Small interfering RNAs (siRNAs) are a class of double-stranded, non-coding RNA molecules, typically 20 to 24 base pairs in length. siRNAs play a role in the RNA interference (RNAi) pathway, interfering with the expression of specific genes by degrading messenger RNA (mRNA), thereby preventing their translation. Conventional siRNAs have the structure of short double-stranded RNAs with a phosphorylated 5' end and a two-nucleotide overhang at the 3' end. siRNAs are catalyzed by the enzyme Dicer to produce them from long double-stranded RNAs and small hairpin RNAs. siRNAs can also be introduced into cells by transfection. siRNA usually triggers gene silencing by a mechanism that consists of four main stages: generation of siRNA, binding to form an RNA-induced silencing complex (RISC), targeting of the mRNA, and degradation of the mRNA.
siRNAs are important biological research tools for gene function validation and drug target screening. In the field of gene silencing, siRNAs can be introduced into cells by transfection to inhibit the expression of specific genes. This property gives it the potential for diagnostic and therapeutic use to address diseases associated with specific genes.
CD BioSciences has a professional technical team ready to provide professional consulting services and assist in solving various problems in experimental design and process. According to the specific needs of clients, we also provide tailor-made siRNA design services to ensure the best experimental results. If you have any questions or needs about our siRNA products or services, please feel free to contact us and we will be happy to serve you.
Our products and services are for research use only and cannot be used for any clinical purposes.
