Bacillus Volatile Antibacterial Agent Preservative

The worldwide transition to natural methods of food preservation has sharpened the search for alternatives to synthetic chemical preservatives. Even though preservatives serve their purpose, they may pose risks in terms of residual poisonous substances, lingering in the environment, and toxicity to the consumers. Volatile bacteriocins derived from Bacillus species have, therefore, come as a new innovative answer because of their antimicrobial activity, environmental safety, and non-toxic property. Bacillus species have the ability to produce volatile organic compounds (VOCs) which have the capability to inhibit pathogenic and spoilage microorganisms. My company is focused on creating specific Bacillus volatile antibacterial preservatives for the food sector, applying biotechnology and green engineering alongside industry needs and safety requirements on a global scale.

Fig 1. Volatile Organic Compounds Produced by Bacillus sp. Strain (Wang Q.Y., 2024)

We offer our bacillus volatile antibacterial agent preservative development service which includes complete preservative formulation design as well as optimization and commercialization based on particular Bacillus strains, Volatile Organic Compounds (VOCs) containing preservatives, and Bacteriocin Preservatives BPC works. Through the use of these spore forming bacilli, we specifically design VOC releasing antifouling preservatives for the food industry with the intention of global commercial use. My services include strain determination, fermentation process refinement and scale up, VOC profiling, stability, and regulatory craftsmanship, ensuring solutions that are both scientifically validated and commercially viable.

Technical Principles

The antimicrobial action of Bacillus volatile organic compounds (VOCs) results from their interactions with the microorganisms of concern on a multifaceted biochemical level. Some of the major mechanisms involved are:

• Membrane Disruption: Surfactin and other lipopeptides destabilize microbial cell membranes. As a result, cellular components are lost, leading to the death of the pathogen.
• Disruption of Energy-Dependent Activities: Specific VOCs causatively impede the vital pathway of ATP production and replication of nucleic acids for spoilage organisms.
• Competitive Inhibition of Adhesion: Certain VOCs bind to specific targeted receptors as competitive antagonists, thus preemptively blocking pathogens from binding to the host surfaces.
• Suppression of Biofilm: Emission of VOCs inhibit the formation of biofilm which is a major biofouling factor in the food processing systems.

Changes to the strain are made through our proprietary techniques, for example, increasing the yield and stability of VOCs using CRISPR-based genome editing. This ensures the efficacy of these compounds across a variety of foods and storage conditions.

Technical Features

• Achieves greater than 99% inhibition of Listeria monocytogenes and Salmonella enterica at very low concentrations.
• Targets bacteria, fungi and enveloped viruses which lessens the need for multiple preservatives.
• Uses strains of Bacillus classified as GRAS (Generally Recognized As Safe) which makes them compliant with international food safety regulations.
• The ability to form spores also aids in withstanding extreme pH, temperature and processing stresses.
• Elimination of harmful residues from food is achieved through the rapid breakdown of VOCs which helps in environmentally friendly food production.

Classification Based on Preservation Categories

Preservative solutions are classified into three groups:

• Wild-type strain solutions: Utilize natural bacteria and Bacillus strains which have VOC production capability ("Bacillus amyloliquefaciens" and "Bacillus Subtilis").
• Engineered strains: Utilize specific pathogen targeting and higher VOC emission rates from genetically enhanced optimally engineered strains.
• Hybrid formulations: Incorporate VOCs with natural synergists like organic acids and plant extracts to solve specific preservation issues in high moisture snacks or acidic beverages.

Application Areas

• Meat and Poultry: Inhibit spoilage bacteria such as Pseudomonas spp. and Brochothrix thermosphacta to extend shelf life.
• Dairy: Control psychrotrophic organisms in cheese, yogurt, and milk products.
• Ready-to-Eat Foods: Prevent cross-contamination during packaging and storage of pre-cooked meals.
• Beverages: Suppress yeast, mold, and bacterial growth in juices, soft drinks, and fermented beverages.

Environmental Benefits

By replacing synthetic preservatives, our solutions mitigate chemical runoff, bioaccumulation risks, and antimicrobial resistance (AMR). Bacillus VOCs degrade naturally without harming ecosystems, while our production processes utilize agricultural byproducts to reduce waste and carbon emissions.

Our Services

We deliver comprehensive support across the preservative development lifecycle:

• Strain Discovery: High-throughput screening of Bacillus strains from diverse environments using genomic and metabolomic tools.
• Fermentation Scale-Up: Optimization of bioreactor conditions to maximize VOC yield and consistency.
• Efficacy Testing: Advanced analytical methods (GC-MS, HPLC) to quantify VOC profiles and validate antimicrobial performance.
• Regulatory Compliance: Preparation of documentation for GRAS, FDA, EUGMP, and other regulatory approvals.
• Custom Solutions: Formulation design tailored to client-specific needs, including compatibility testing with food matrices.

Company Service Advantages

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In response to increasing demand for environmentally friendly food preservatives, our bacillus volatile antibacterial agent development service offers a highly effective, scientifically grounded solution that replaces traditional preservatives. These technologies, alongside our decades-long research into microorganisms, enable us to improve food security while sustaining the health of the planet. Join us in transforming the future of food preservation sustainably, effectively, and through innovative technologies.

How to Place an Order

Our products and services are for research use only and cannot be used for any clinical purposes.