Multi-Stage Fermentation
The global food industry faces a serious food waste problem. Landfilling contributes to GHG emissions and resource depletion. CD BioSustainable offers multi-stage fermentation services using high-pressure thermal pretreatment (HPTP) and Yarrowia lipolytica yeast to decompose food waste, separate oil and grease, and produce protein-rich yeast biomass, supporting circular economy in the food sector.
Fig 1. Multi-Stage Fermentation (Wang X.L., 2024)
Technical Principles
- High-Pressure Thermal Pretreatment (HPTP): Food waste is treated with heat and pressure to disinfect and hydrolyze starches and proteins into simpler forms, while separating and liquefying oils and greases from the waste matrix.
- Biological Treatment with Yarrowia lipolytica: The pretreated hydrolysate is used to cultivate Yarrowia lipolytica, an oleaginous yeast that assimilates fats and sugars, purifying the waste stream and producing protein- and lipid-rich yeast biomass. Separated oils from HPTP can also be valorized or utilized by the yeast.
Technical Features
- Resource Valorization: This process enables the recovery of valuable oils and fats and the generation of yeast protein biomass suitable for animal feed or other bio products.
- Pathogen Destruction: HPTP stage guarantees complete sterilization by eliminating the population of pathogens, thus lowering biosecurity threats.
- Robust Microbial System: Yarrowia lipolytica has a high tolerance to many inhibitory substances that are frequently present in food waste and has a broad range of substrates.
- Reduced Waste Volume: Reduction in the amount of waste that requires disposal is very significant.
Application Areas
- Food and beverage manufacturing plants
- Large-scale catering facilities and restaurants
- Supermarkets and food distribution centers
- Agricultural processing residues
- Municipal organic waste management programs with source-separated food waste.
Environmental Benefits
- Reduced Landfill Burden: Diverts significant quantities of organic waste from landfills.
- Lower Greenhouse Gas Emissions: Prevents methane generation associated with anaerobic decomposition in landfills.
- Circular Economy Promotion: Converts waste into valuable resources, closing the loop on nutrient and material flows.
- Reduced Water Pollution: Effective treatment reduces the pollutant load of wastewater.
Our Services
Understanding that each client's needs and waste streams are unique, we offer a suite of tailored services designed to ensure successful implementation and operation of our multi-stage fermentation technology. Our commitment extends beyond mere technology provision to encompass a full lifecycle partnership. We can provide:
- Feasibility Studies and Waste Stream Analysis: Comprehensive assessment of your specific food waste streams, including characterization and treatability studies to determine the optimal process parameters.
- Pilot-Scale Testing: On-site or off-site pilot trials to demonstrate process efficacy and gather data for full-scale design.
- Custom Process Design and Engineering: Tailoring the multi-stage fermentation system to your specific throughput requirements, site constraints, and valorization goals.
- Process Optimization and Monitoring: Continuous monitoring and data analysis services to optimize performance and maximize resource recovery.
- By-product Valorization Consultation: Guidance on potential markets and applications for the recovered oils and yeast biomass.
Contact Us
We partner with the food industry to implement sustainable solutions for a cleaner planet and circular economy. Contact us for more information.
How to Place an Order
Reference
- Wang X.L., Sun Y.Q., et al. "Kinetics-based development of two-stage continuous fermentation of 1,3-propanediol from crude glycerol by Clostridium butyricum" Biotechnology for Biofuels and Bioproducts 2024, 38:2580.
Our products and services are for research use only and cannot be used for
any clinical purposes.
