Enteric Fermentation Base
Enteric Fermentation Base

Catalog Number FM-2297

Product Name Enteric Fermentation Base

Size 250 g

Product Information

Application Used to identify the fermentation reactions of various aerobic bacteria to sugars.
Principle Beef paste powder and peptone provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride maintains a balanced osmolality; disodium hydrogen phosphate is a buffer; muskoxylan bromide is a pH indicator, yellow for acidity and blue for alkalinity; and a variety of sugars are added to the basal medium to test for the characterization of the bacterial fermentation of sugars.
pH 7.4±0.2
Formulation (/L) Peptone 10.0 g
Beef baking powder 5.0 g
Sodium chloride 3.0 g
Disodium hydrogen phosphate 2.0 g
Bromo muscovite blue 0.025 g
Directions Weigh 20g of this product, add 5g of sugar, add distilled water or deionized water or deionized water 1 L, heating and stirring to dissolve and boil until completely dissolved, divided into small inverted tubes in test tubes, 121°C autoclave sterilization for 15min. some sugar (sucrose, lactose, xylose, arabinose, cellobiose, etc.) will be hydrolyzed when heated, it should be dissolved, filtered and sterilized, and then added to the test tubes equipped with the basic medium has been sterilized, so that the final concentration of sugar is 0.5 mg of sugar. Make the final concentration of sugar 0.5%.
Shelf Life 3 years

Storage

Store in a dry place away from light and tighten the cap immediately after use.

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