Keep in airtight containers below 30°C. Prepared media is kept at 2-8°C.
Modified Duncan Strong (DS) Medium 
Catalog Number FM-2269
Product Name Modified Duncan Strong (DS) Medium
Aspect Creamy to yellow powder
| Application | Used for isolation and differentiation of Clostridium perfringens from foodstuffs on the basis of cotton seed sugar fermentation. |
| Principle | Proteose peptone and yeast extract supply nitrogenous compounds and other nutrients. Sodium thioglycollate ensures anaerobic conditions. Disodium phosphate acts as a buffer. Raffinose is specifically fermented by C. perfringens to produce acid within 72 hours. Acid production is tested by adding bromothymol blue, turning the medium yellow. |
| pH | 7.8±0.2 |
| Colour and Clarity of Prepared Medium | Yellow clear solution |
| Formulation (/L) |
Proteose peptone 15.0 g Yeast extract 4.0 g Sodium thioglycollate 1.0 g Disodium phosphate 10.0 g Raffinose 4.0 g |
| Directions | Add 34 g to 1000 ml distilled water and mix well. Heat if necessary to dissolve the medium completely. Autoclave at 15 pounds pressure (121°C) for 15 minutes. Dispense into sterile tubes. Check one or both tubes to measure pH. |
Keep in airtight containers below 30°C. Prepared media is kept at 2-8°C.
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