• Mycothiazole is a mitochondrial electron transport chain (ETC) complex I inhibitor, showing cytotoxic effects on cancer cells such as Huh7 (IC₅₀ = 55.8 μM), U87, and MCF7. It induces apoptosis in Huh7 cells and utilizes the unfolded protein response (UPR) and heat shock response (HSR) pathways, involving transcription factors ATFS-1 and HSF1, to extend the lifespan of C. elegans.