Neuroinflammatory-IN-1

Neuroinflammation, the inflammation of nervous tissue, stands as a critical pathological driver behind both acute brain insults (e.g., stroke, trauma, haemorrhage) and chronic neurodegenerative conditions including Alzheimer’s disease, Parkinson’s disease, and multiple sclerosis. This process is initiated by cues like infection, toxic metabolites, or autoimmunity, triggering the activation of microglia—the resident innate immune cells of the central nervous system (CNS). When hyperactivated, microglia release excessive proinflammatory mediators, compromising the blood-brain barrier (BBB) and impairing neuronal survival. Among these mediators, nitric oxide (NO) plays a pivotal role in propagating inflammatory damage, making its inhibition a key target for anti-neuroinflammatory intervention.
Against this backdrop, Neuroinflammatory-IN-1 (also referred to as Compound 5 in scientific literature) emerges as a specialized bioactive agent. Classified under phenylpropanoids and coumarins—structural families with well-documented biological activities—it is originally derived from plants of the Umbelliferae family. With the CAS number 83988-97-0, a molecular formula of C₂₄H₃₀O₄, and a molecular weight of 382.49, this compound is engineered to target neuroinflammatory pathways at a molecular level. Its core functionality lies in inhibiting NO production, with an IC₅₀ value of 65.4 μM, positioning it as a valuable tool for dissecting NO-mediated inflammatory processes in the CNS.

Delivers actionable biological data: The IC₅₀ value of 65.4 μM for NO inhibition provides a quantifiable benchmark for comparing anti-neuroinflammatory efficacy in experimental models.
Derived from natural plant sources (Umbelliferae family), appealing to researchers focusing on natural product-based anti-inflammatory therapies—a growing area of neuropharmacology.
Supports reproducible research: Its well-documented chemical structure and purity (backed by analytical certificates) minimize variability across experiments.
Compatible with standard laboratory workflows, including cell-based assays targeting microglia or other CNS cell types involved in inflammation.
Complements existing research tools: Aligns with studies on cytokine-mediated inflammation (e.g., IL-1, TNF-α) by targeting a parallel NO-dependent pathway.

In vitro studies on neuroinflammatory mechanisms: Ideal for investigating the role of NO in LPS-induced or cytokine-mediated (e.g., IL-1β) inflammation in microglial, astrocyte, or brain endothelial cell lines.
Evaluation of blood-brain barrier (BBB) protection: Can be used to assess whether NO inhibition mitigates BBB leakage— a common consequence of neuroinflammation—in tri-culture or co-culture models.
Screening for synergistic anti-neuroinflammatory combinations: Compatible with combination studies involving other bioactive compounds (e.g., andrographolide, baicalein) to explore enhanced efficacy against proinflammatory mediators like IL-6 and TNF-α.
Research on chronic neurodegenerative models: Supports investigations into how NO suppression impacts disease progression in in vitro systems mimicking Alzheimer’s, Parkinson’s, or multiple sclerosis.
Validation of NO-dependent signaling pathways: Useful for confirming the role of NO in downstream inflammatory events, such as glial activation or neuronal cell death.

In the field of anti-neuroinflammatory research, complementary products include signaling pathway inhibitors like NF-κB inhibitor Bay 11-7082 (which modulates cytokine-driven inflammation) and p38 MAPK inhibitor SB 203580 (targeting stress-activated inflammatory cascades). Natural compounds with neuroprotective properties, such as curcumin and resveratrol, are also widely used for their anti-inflammatory and antioxidant effects in CNS models. Additionally, cytokine modulators like IL-1 receptor antagonist (IL-1ra)—which targets the pivotal IL-1-mediated inflammatory pathway—and TNF-α inhibitors are essential tools for dissecting cytokine-specific neuroinflammatory responses. If you are interested in related products, you can directly contact us or inquire about product customization services.